Salmonella Detection Kit

MAMBA® orange line – Identification of Bacteria

This qPCR Salmonella spp. detection kit targets the tetrathionate reductase subunit A (ttrA) gene from Salmonella spp. This group is devided into two species: S. enterica (with six subspecies and more than 2500 known serovars) and S. bongori.

Salmonella is a bacterium which primarily infects poultry and livestock but can also infect humans and other animals. Infection can lead to salmonellosis (gastroenteritis orenteric typhoid fever). Therefore detection of this bacteria is important for animal and human health by testing food and feed products. To provide a meaningful test we provide an internal control to minimize false results. This kit detects Salmonella spp. via a specific probe which can be measured in the FAM channel after amplification. It provides anything for the Real-Time-PCR as well as analysis and interpretation. The internal control is detected via hex channel. We advise to use the protocol DIN EN ISO 10135, May 2013 for the cultivation and enrichment as well as the extraction of DNA.

The Kit is designed for 96 reactions with a final reaction volume of 20 µl including at least one positive and one negative control.

  • Detects all Salmonella species and serotypes
  • Detection based on cultivation via DIN 10135
  • REF: OR-001-96x
Data sheet
Workflow
Manual

Applicability

  • Compatible with lowand high-throughput qPCR instruments.
  • Validated with BioRad CFX ConnectTM, Applied Biosystem 7500 FAST, QuantStudioTM
  • Utilizes conventional FAM and HEX marked probes.
  • Multiplex approach. Each well contains an internal control to detect sample inhibition.
  • Shelf life: 1 year at −20 °C or 6 months at 4 °C.
  • Includes 5 freeze and thaw cycles
  • Two pipetting steps required to prepare the ready-to-use PCR mix, the risk of contamination is significantly reduced.
  • Results in less than 1.5 hours

Specificity

Detects all Salmonella species and
serotypes.
Tested against a various number of
closely related bacteria and DNAs
relevant in animal feed.

Exclusivity

Target DNA at low levels (≈5 copies)
was successfully detected in mixed
samples containing high levels of
non-target bacterial DNA (≈ 5000
copies).

Sensitivity

  • Capable of detecting up to 5 copies of Salmonella plasmid DNA, even in the presence of a high background flora.
  • Different numbers of copies of the target were tested and can be seen in the graph on the right.
    • 1000 copies (left), 100 copies (middle), 5 copies (right)
    • red = Salmonella spp.
    • blue = Internal control

Reliability

  • System remains stable and robust in relation to temperature (±1 °C), mastermix volume (-6 %), probe and primer concentration (-30 %) variations
  • The system was tested under standard (60 °C) and tripel altered conditions (59 °C, -6 % volume, -30% primer ans probe conzentration).
    • red = Salmonella spp.
    • blue = Internal control