Identification of Genetically Modified Plants

MAMBA® green line – Identification of Genetically Modified Plants

The number of different bacterial species can only be roughly estimated, but millions of them probably exist on earth. Many are essential for life, but some are extremely dangerous, e.g., foodborne pathogens such as Salmonella spp. or Listeria monocytogenes.

No microbiological food testing is complete without these two species.

MAMBA DIAGNOSTIK provides complete qPCR-kits for the specific and sensitive identification of this important pathogens.

Salmonella Detection Kit

  • Detects all Salmonella species and serotypes
  • Detection based on cultivation via DIN 10135
Pipettierschema_qPCR Kit SAL_06Herunterladen

Listeria Detection Kit

  • Detects all Listeria species and serotypes
  • Additionally detects the V139E variant
Pipettierschema_qPCR Kit LIS_01Herunterladen

Other Detection Kit

  • Other detection kits available on demand
  • By interest in other species detection kits please
    contact us: info@mamba-diagnostik.de
Pipettierschema_Extraktionskit_ 01Herunterladen

GVO

  • Double screening (DNA-Marker „35 S-Promotor“ and „Nos-Terminator“)
  • GTS-40-3-2/Roundup Ready I-Soja

Applicability

  • Compatible with lowand high-throughput qPCR instruments.
  • Validated with BioRad CFX ConnectTM, Applied Biosystem 7500 FAST, QuantStudioTM
  • Utilizes conventional FAM and HEX marked probes.
  • Multiplex approach. Each well contains an internal control to detect sample inhibition.
  • Shelf life: 1 year at −20 °C or 6 months at 4 °C.
  • Includes 5 freeze and thaw cycles
  • Two pipetting steps required to prepare the ready-to-use PCR mix, the risk of contamination is significantly reduced.
  • Results in less than 1.5 hours

Specificity

Detects all Salmonella species and
serotypes.
Tested against a various number of
closely related bacteria and DNAs
relevant in animal feed.

Exclusivity

Target DNA at low levels (≈5 copies)
was successfully detected in mixed
samples containing high levels of
non-target bacterial DNA (≈ 5000
copies).

Sensitivity

Capable of detecting up to 5 copies of Salmonella plasmid DNA, even in the presence of a high background flora.

Sensitivity results

Multiple copy numbers tested: 1000 (left), 100 (middle), 5 (right)

  • red = Salmonella spp.
  • blue = internal control

Reliability

System remains stable and robust in relation to temperature (±1 °C), mastermix volume (-6 %), probe and primer concentration (-30 %) variations

Reliability results

Tested under standard (60 °C) and triple altered
conditions (59 °C).

  • red = Salmonella spp.
  • blue = internal control